The Niche Architecture and Structure of Cancer Stem Cells

Stem cell niche

Niche in tumor - low power not shown in poster (see also high power: AACR_Scan01.jpg)

Stem cell niche

Niche in tumor (Mild intraepithelial neoplasia of uterine cervix) see also high power: AACR_Scan02.jpg

Stem cell niche progenitor cell

Stem cell niche with progenitor cell (Infiltrating carcinoma of uterine cervix - CIS)

Stem cell niche halo

Stem cell niche: Halo formation between inner and outer membrane. (Medullary carcinoma of breast)

Stem cell niche halo

(see also low power: AACR_Scan05.jpg , DSC_1475.jpg) Stem cell niche: Cell in quiescent state; halo formation between inner and outer membrane; niche surrounded by closely attached support cells. (High-grade intraepithelial neoplasia)

Stem cell niche halo

(see high power: AACR_Scan04.jpg) High-grade intraepithelial neoplasia of uterine cervix (duplicate: DSC_1475.jpg)

cervix - Infiltrating squamous cell carcinoma - Niche with asymmetric division

(see high power 01120001.jpg) Niche surrounded by support cells with one self-renewal daughter cell (arrow) consisting of spheres and one differentiated tumor cell. (Infiltrating squamous cell carcinoma of uterine cervix [2])

cervix - Infiltrating squamous cell carcinoma - Niche with asymmetric division

(See also: DRNEPT1-R01-014.Jpg) Niche surrounded by support cells with one self-renewal daughter cell consisting of spheres and one differentiated tumor cell. (Infiltrating squamous cell carcinoma of uterine cervix [2])

Asymmetric division papillary adenocarcinoma uterine cervix

Asymmetric division: Self-renewal daughter cell and progenitor cell consisting entirely of spheres with uniform luminal diameters. (Papillary adenocarcinoma of uterine cervix [3]) (duplicates: 01120006.jpg

Asymmetric division papillary adenocarcinoma

(see high power: DSC_1470.jpg; see also: 01120007.JPG ) Asymmetric division: Self-renewal daughter cell and progenitor cell consisting entirely of spheres with uniform luminal diameters. (Papillary adenocarcinoma of uterine cervix [3])

Niche self-renewal daughter cell

(see low power: 1459): abandoned niche: may be remains of self renewal cell) Niche with single self-renewal daughter cell. (Papillary carcinoma of urinary bladder)

Epithelial cells G0 G1 G2

Epithelial cells: Complete absence of spheres with the exception of sporadic spheres in tumor cells

Stem cell migration

(see high power: AACR_Scan15.crop) Migrating stem cell with rugged border. Cell consists of uniform-sized spheres. (Adenocarcinoma colon)

Stem cell migration

(see low power: AACR_Scan16.crop) Migrating stem cell with rugged border. Cell consists of uniform-sized spheres. (Adenocarcinoma colon)

Stem cell migration

(see high power AACR_Scan17.jpg) Migrating stem cell: Cell consists of uniform-sized spheres. (Adenocarcinoma colon)

Migrating stem cell

(see low power AACR_Scan18-crop-circle.jpg) Migrating stem cell: Cell consists of uniform-sized spheres. (Adenocarcinoma colon)

Migrating stem cell

(see low power DRNEPT2-R01-026-circle.Jpg) Migrating stem cell : Cell consists of uniform-sized spheres. (Benign area adjacent adenocarcinoma colon)

Migrating stem cell blood

Migrating stem/progenitor cell with rugged border. (Blood smear from patient with breast cancer)

Migrating stem cell blood

Migrating stem cell with rugged border. (Blood smear from patient with uterine cervix cancer)

Buccal mucosa spread

Buccal mucosa: malignancy-associated nuclear structure Prominent spheres with uniform diameters. (smear from patient with squamous cell carcinoma lung)

Buccal mucosa spread

Buccal mucosa: malignancy-associated nuclear structure of prominent spheres with uniform diameters (from patient with colon adenocarcinoma)

buccal

Prominent spheres with uniform diameters. (Patient with ovarian cancer)

buccal

Prominent spheres with uniform diameters. (Patient with recurrent mucus cyst adenocarcinoma) see also: DRNEPT3-R01-004.jpg

buccal mucossa spread - gastric adenocarcinoma

Buccal mucosa: malignancy-associated nuclear structure - spheres of varying size (smear from patient with gastric carcinoma)

Circulating leukemic DNA-induced spheres

Prominent spheres with uniform diameters similar to buccal cells. Circulating leukemic DNA-induced spheres -Anker study: same culture as DSC_1478.jpg. (see duplicate: DSC_1484.jpg)