ISPO

Published in Cancer Detection and Prevention 2004; 28(4).

RET polymorphisms in codons 769 and 836 are not associated with predisposition to medullary thyroid carcinoma

Malgorzata Wiench, PhDa,, Jan Wloch, MDb, Zbigniew Wygoda, MDa, Elzbieta Oubala, PhDa, M. Oezkoa, A. Pawlaczeka, D. Kulaa, D. Lange, MD c, B. Jarzab, PhDa

aDepartment of Nuclear Medicine and Endocrine Oncology Maria Sldodowska-Curie Memorial Institute, 44-101 Gliwice, Poland; bClinic o/'Ontological Surgery, Maria Sklodowska-Curie Memorial Institute, 44-101 Gliwice, Poland ; cDepartment of Pathology Center of Oncology, Maria Sklodowska-Curie Memorial Institute, 44-101 Gliwice, Poland

The study was undertaken to verify whether the RET gene polymorphisms are associated with MTC in patients negative for germline mutations. Two hundred five patients with apparent sporadic MTC were subjected to genetic analysis of RET exons 10, 11, 13, 14, 16 and 22 RET germline mutation carriers were identified with 10.7% frequency. The frequency among 26 patients not older than 30 was 27%. In patients excluded for known mutations we analyzed two polymorphic sites: RET codon 769 and 836. As control group, 90 healthy subjects were investigated. In young patients the observed allelic frequencies were 32% for variant L769/CTG and 5% for variant S836/AGT. Although these values were higher than in older MTC patients (22 and 3%, respectively), as well as in the control group (27 and 2%) the difference was insignificant. We conclude that in Polish patients polymorphisms at RET codons 769 and 836 are not associated with medullary thyroid carcinoma.

KEY WORDS: RET protooncogene, Polymorphism.

http://www.cancerprev.org/Journal/Issues/28/4/4895