Published in Cancer Detection and Prevention 2002; 26(6).

Close correlation between restriction fragment length polymorphism of L-myc gene and susceptibility to gastric cancer

Turgay Isbir, PhDa, Ilhan Yaylim, PhDa, Soykan Arikan, MDb, Esra Kaytan, MDc, Tamer Karşidağ, MDb, Savaş Bayrak, MDd, Hakan Çamlica, MDe

aDepartment of Molecular Medicine, Institute of Experimental Medical Research, University of Istanbul, DETAE 34390, P.O. Box 7 Çapa, Istanbul, Turkey bHaseki Research and Educational Hospital, Surgery Clinic, Istanbul, Turkey cInstitute of Oncology, University of Istanbul, Istanbul, Turkey dSSK. Istanbul Education Hospital, Surgery Clinic, Istanbul, Turkey eInstitute of Oncology, Division of Cancer Epidemiology and Bioistatistics, University of Istanbul, Istanbul, Turkey

AIM: A common inherited RFLP of the L-myc proto-oncogene has been reported to correlate with cancer susceptibility. Our aim was to test the hypothesis that there was association between L-myc S allele in gastric cancer and predisposition to the disease. METHODS: The distribution of L-myc polymorphism in 25 patients with gastric cancer was determined by polymerase chain reaction-based restriction fragment length polymorphism and compared with that of 83 healthy control subjects. RESULTS: We found a significant difference, both in the distribution of the LL, LS and SS genotypes and in the allelic frequencies, between the control group and the patient group; that is, the frequencies of L-myc alleles were, L and S, 0.52 and 0.48, 0.64 and 0.36, respectively. This difference was primarily the result of a high frequency of the S allele among gastric cancer patients compared to controls. There was a significant difference in the distribution of both genotypes ( P = 0.004) and allele frequencies (P = 0.005) between patients with gastric cancer and control groups. CONCLUSIONS: Our results suggested that L-myc polymorphism may be significant in an individual's susceptibility to gastric cancer in Turkey and may be useful for identifying patients at high risk of developing gastric cancer.

KEY WORDS: L-myc, Gastric cancer, Polymorphism.