Immunopathological study of secretory Iga expression in helicobacter pylori-infected gastric mucosa

KD Dimopoulou, E. Kairi-Vassilatou, M. Frangou, PhD, M.Eglezou, K. Kontoyanni MD, A. Kondi-Pafiti

Areteion Hospital, Athens University Medical School-Greece, Athens, Attica Greece

AIM: Aim of the study is to investigate the distribution of Secretory Component- IgA immunoprotective system in gastric mucosa with inflammatory changes, associated with helicobacter pylori infection. Material-Method. 45 gastric biopsies with moderate (25/45) and severe (20/45) inflammatory changes were investigated by immunohistochemical method for helicobacter pylori ( mab Biosoft, France), Iga and Secretory component (mabs DaKo, Denmark). Results. In 14/45 biopsies (31.8%) H.Pylori +ve immunoreaction was noted at the mucosal surface and in the mucous. 3/ 14 specimen presented a glandular +ve reaction for secretory component , located on the apical border or the cytoplasm .A IgA+ve reaction was noted in 12/14 cases, located in the plasma cells of the lamina propria .In 21/ 31 non- H.Pylori associated gastritis, a extensive and intense +ve reaction for Secretory Component was noted, located mainly at the apical border, the cellular membranes and in the glandular secretions. In 3/31 cases a cytoplasmic location of Secretory Component was noted. All cases presented a variable +ve IgA reaction in the plasma cells and in the glandular secretions. In conclusion, there is evidence that the presence of H.Pylori in the gastric mucus affects the glandular cell production of Secretory Component and the synthesis of the Secretory-IgA molecule. This results in decreased protective action of the gastric local immunoprotective system.

KEY WORDS: Helicobacter Pylori, Secretory Component, IgA, Gastritis.

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Paper presented at the International Symposium on Predictive Oncology and Intervention Strategies; Paris, France; February 9 - 12, 2002; in the section on Immunotherapy.