cDNA responses of panc-1 pancreatic and H-358 lung cancer cells to the 5-lipoxygenase inhibitor, MK 886 and panc-1 cells to cisplatin

KM Anderson, md,PhD

Rush Medical College and Rush Presbyterian St. Luke's Med Center, Chicago,, IL

AIM: To (a) compare the cDNA responses of Panc-1 pancreatic cells and H-358 lung cancer cells to the 5-lipoxygenase inhibitor, MK 886 and (b), compare the Panc-1 response to cisplatin. METHODS: Procedures for Clontech human cDNA arrays (Palo Alto, CA) were followed. RESULTS: (a) cDNA responses of Panc-1 and H-358 cells to MK 886 were similar, with evidence for reduced proliferation and increased countervailing and other stress response events, including extensive mRNA antioxidant, heat shock with limited DNA repair. (b) Panc-1 responses to cisplatin and MK 886 revealed similarities (inhibition of proliferation, increased countervailing events) and differences (less oxidative stress, more extensive DNA repair). CONCLUSIONS: (1) mRNA responses to MK 886 in 2 different cell lines were comparable. (2). Significant differences between the response of Panc-1 cells to MK 886 or cisplatin were evident. (3) Therefore, relatively simple cDNA arrays can identify and distinguish major anti-proliferative, countervailing, cellular stress and programmed cell death mRNA - responses. (4) The extent to which relatively unselective agents induce countervailing genomic responses is under-appreciated.

KEY WORDS: cDNA array, Panc-1, H358 responses.

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Paper presented at the International Symposium on Predictive Oncology and Intervention Strategies; Paris, France; February 9 - 12, 2002; in the section on Gene Expression, Part 2.