Detection of oncogenic HPV DNA sequences in sporadic retinoblastoma

MA Palazzi,MDa,JA Yunes,PhDa,IA Cardarelli,MDa,G Stangenhaus,PhDa,SR Brandalise,MDa,SA Ferreirab,JSP Sobrinhob, LL Villa,PhD b.

aBoldrini's Children Center,Campinas,Sao Paulo Brazil. bDepartment of Virology, Ludwig Institute for Cancer Research, Sao Paulo, Brazil

AIM: Evaluate the presence of HPV DNA in paraffin-embedded tumor tissue from patients with sporadic retinoblastoma. METHODS: Four micrometer slices of each block were cut and stained with H&E for histopathological review. Twelve micrometer slices were sectioned from the tumor area of each block for DNA extractions and HPV DNA typing by generic PCR with GP5+/GP6+ primers. HPV type specific identification was done by dot blot hybridization. All cases were tested for DNA adequacy by globin amplification. RESULTS: HPV DNA was found in 12 out of 43 retinoblastomas(27,9%). HPV DNA type 16 was found in 10 tumor specimens and HPV 35 was found in 2. One tumor had HPV DNA of both types(16 and 35)and in another case, an undetermined HPV type was detected. CONCLUSIONS: Some sporadic retinoblastoma contain oncogenic HPV DNA. However, any role of HPV in the genesis of these tumors awaits further investigations.

KEY WORDS: Human papillomavirus, polymerase chain reaction, sporadic retinoblastoma.

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Paper presented at the International Symposium on Predictive Oncology and Intervention Strategies; Paris, France; February 9 - 12, 2002; in the section on Familial and Hereditary Cancer.