Diallyl disulfide induces STAT1 gene expression in human colon tumor cells (colo 205)

JG Chung, PhDa, WL Wanga, HF Lub, GW Chen PhDc, HC Ho PhDd

Department of Micrbiologya and Biochemistryd, China Medical College, Taichung, Taiwan ROC. Department of Urologyb and Surgeryc, China Medical college Hospital, Taichung, Taiwan ROC

Diallyl sulfide (DADS), a thioether compound found naturally in garlic, was evident by its ability to inhibit the growth of human colon tumor cells. The mechanism by which DADS inhibits the growth of human colon tumor cells has not been fully understood. Here we report that DADS increased the gene expression of STAT1 on colo 205 cells, a human colon adenocarcinoma cell line. The gene expression of STAT1 in colo 205 cells was examined and determined by using two different methods (differential display RT-PCR and flow cytometry). The data demonstrated that DADS induced STAT1gene expression is dose- and time-course dependent effect. Moreover, fas receptor is critical for the induction of STAT1 expression by DADS. Because added inhibitor ((-)-epigallocatechin gallate) to cells led to decrease the levels of STAT1. These results indicate that STAT1 gene is activated by DADS in human colon tumor cells and these effects are underwent the fas receptor.

KEY WORDS: human colon tumor cells, .

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Paper presented at the International Symposium on Predictive Oncology and Intervention Strategies; Paris, France; February 9 - 12, 2002; in the section on Dietary Influences.