Circadian periodicity of plasma lipid peroxides and other antioxidants as putative markers in gynecological malignancies

R. Singh, MDa, R.K. Singh, MDa, A.A. Mahdi, MDa, A. Kumar, MDa, A.K. Tripathi, MDb, A. Chandra, MDa, R.K. Singh, MDa , U. Singh, MDc , S. Misra, MDd, G. Cornelissen, PhDe , F. Halberg, MDe

Departments of aBiochemistry, bMedicine, cObstetrics & Gynecology, dSurgical Oncology, King GeorgeÕs Medical College, Lucknow Ð 226003, India; and e Halberg Chronobiology Center, University of Minnesota, 420 Delaware St. S.E., Minneapolis, MN 55455 USA.

AIM: Lipid peroxidation and antioxidant defense mechanisms may relate to preventive and curative chronochemotherapeutic efficacy and management. METHODS: 30 women with newly diagnosed gynecological malignancies (mainly carcinoma of cervix and ovary), 30 to 60 years of age group and 35 age-matched clinically healthy women were synchronized for one week with diurnal activity from about 06:00 to about 22:00 and nocturnal rest. Breakfast was around 08:30, lunch around 20:30. Drugs known to affect the free radical system were not taken. Determinations included plasma lipid peroxides, in the form of malonyl dialdehyde (MDA, an index of lipid peroxidation), blood superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GR) activities, and serum ascorbate, urate and high density lipoprotein cholesterol (HDL-C) concentrations. RESULTS: A marked circadian variation was demonstrated for each variable in each group by population-mean cosinor (P<0.002). In addition to anticipated differences in overall mean value (MESOR), patients differed from controls also in terms of their circadian pattern. Mapping the broader time structures (chronomes) with age and multifrequency rhythm characteristics of antioxidants and prooxidants is needed for exploring their putative chemotherapeutic role as markers in cancer chronoprevention and management of the disease.

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Paper presented at the International Symposium on Predictive Oncology and Intervention Strategies; Paris, France; February 9 - 12, 2002; in the section on Prevention.