8-hydroxydeoxyguanosine mediates cell death of KG-1 by inducing apoptosis

JW Hyun, PhD, MH Chung, PhD, MD

Department of Pharmacology, Seoul National University College of Medicine, Seoul, Korea Republic

Recently, we have reported that 8-hydroxydeoxyguanosine shows the cytotoxicity on KG-1. In this study, 8-hydroxydeoxyguanosine mediated cytotoxicity is attributable to the induction of apoptosis. The apoptosis was evidenced by increase of sub G1 hypo-diploid cells, DNA fragmentation, and morphological apoptotic body formation. This apoptotic process was associated with down-regulation of bcl-2 and formation of cleaved bid by caspase 8, loss of mitochondria transmembrane potential, cytochrome c, caspase 9 and caspase 3 activation. These findings suggest that hydroxydeoxyguanosine mediated cytotoxicity of KG-1 by the induction of apoptosis.

KEY WORDS: Mitochondria transmembrane potential, Cytochrome c, Caspase activation.

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Paper presented at the International Symposium on Predictive Oncology and Intervention Strategies; Paris, France; February 9 - 12, 2002; in the section on Apoptosis - Molecular Mechanisms.