Distinctive in chromosome numbers and cell cycle response to cytokines were observed between paired primary and metastatic melanoma cell lines

A.R.Andaliba, M. Salehia, K. Sisleyb, J. Lawryb

aIsfahan Medical School-Isfahan-Iran , Institute for Cancer Studies, bSheffeild Medical School-UK

Cell cycle and cytogenetic analysis were performed in a group of paired primary and metastatic melanoma cell lines following standard protocols. Tissue culture facilities and flow-cytometry technique were applied for assessment of the effect of several cytokine in tumour cell cycle. The paired cell lines WM793 and WM1205 had chromosome counts of 46-52 and 47-57 respectively, both had a modal count of 51 and the lines shared common chromosome abnormalities. The pattern of the effect of IFN-alpha and IFN-gamma on the cell cycle of metastatic paired (WM1205) was different compare to the primary counterpart (WM793). Pair WM983A and WM983B have chromosome counts 52-63 and 50-66 respectively, and modal values of 54 and 61 respectively. Alteration of chromosome 6, 9 and 12 were common in both lines. However, alteration in respect to IL-1-beta, IL-6, IFN-gamma in the cell cycle events of metastasis line was observed. Analysis of WM1361A and WM1361C showed the lines had chromosome counts ranging from 60-63 and 47-68 with modal counts of 60 and 57 respectively. The lines appeared to share common abnormality including alterations of chromosomes. The responsiveness of the metastatic line (WM1361C) to TNF-alpha, TGF-beta2, and IFNs was different with its primary counterpart (WM1361A). The alteration in responsiveness to cytokines in metastatic cell lines compared to their primary counterparts could be caused by the alteration in chromosomes in the metastatic lines.

For more information, contact

Paper presented at the International Symposium on Predictive Oncology and Intervention Strategies; Paris, France; February 9 - 12, 2002; in the section on Metastasis.