Benzopyrene-diol-epoxide (BPDE)-DNA adducts analysis in a group of cervical carcinoma patients from Bangladesh. Relationship with carcinogen differential exposure

Ana Dardeli M.D1., Shahadat Hossain, M.D.,2, Alma Boninsegna, Ph.D,1, Giovanna Flamini, Ph.D.,1, Giovanni D'Abramo Ph.D.3 and Achille Cittadini, M.D,1

1 Institute of General Pathology, Giovanni XXIII Cancer Center, Catholic University School of Medicine, Largo F. Vito, 1-00136, Rome., Italy. Email: 2 Bangladesh Cancer Institute, New Market, Dhaka-1205, Bangladesh. Email: 3 Institute of Physics Catholic University School of Medicine, Largo F. Vito, 1-00136, Rome., Italy,

AIM: a group of 50 women from Bangladesh carrying invasive cervical carcinoma has been studied with the aim to evaluate Benzopyrene-DNA adducts in relation with the consumption of paan-zarda, a complex mixture constituted of Betel, Areca, and dried tobacco leaves. The exposure period ranged from 3 up to 9 years. METHODS: Exposure to Benzopyrene was evaluated analyzing by immunohistochemistry Benzopyrene-diol-epoxide (BPDE)-DNA adducts in exfoliated cervical cells; passive smoking exposure and HPV (16 and/or 18) infection were also taken in consideration. The positive nuclei were counted and scored for intensity. Such marker was statistically compared by the Tau Kendall test. RESULTS: adducts intensity resulted significantly related with: 1) cancer disease (p=0.03), 2) paan plus zarda chewing (p=< 0.00001) and 3) period of chewing (p=< 0.00001). Moreover, a significant relationship was found between HPV 16 positivity and paan plus zarda chewing (p=0.008). No significant results were obtained comparing passive smoking exposure with adducts positivity. CONCLUSION: it may be suggested that adducts are a good marker to demonstrate the involvement of tobacco exposure by chewing in cervical cancer development. A confirmation of such observation is given by the non significance in carcinogenesis of paan chewing or passive smoking exposure. From the overall data it can be confirmed that HPV infection, given its ability to immortalize cervical cells, allows carcinogen exposure to induce carcinogenesis.

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Paper presented at the International Symposium on Predictive Oncology and Intervention Strategies; Paris, France; February 9 - 12, 2002; in the section on Carcinogenesis.