Published in Cancer Detection and Prevention 2001; 25(3):301-308.

Allelotype of the Adenoma-Carcinoma Sequence of the Stomach

Hee Sung Kim,a Dong Kyun Woo,b Soo In Bae,a Yong Il Kim,a Woo Ho Kima,b

aDepartment of Pathology and the bCancer Research Institute, Seoul National University College of Medicine, Seoul, Korea.

Address all correspondence and reprint requests to: Address correspondence and reprint requests to: Woo Ho Kim, MD, Department of Pathology, Seoul National University College of Medicine, 28 Yongon-dong, Chongno-gu, Seoul 110-799, Korea.

ABSTRACT: In order to identify the significant allelic loss involving the gastric adenoma-carcinoma sequence, we used 49 genome-wide microsatellite markers in an allelotype study of 30 cases of stomach resections that harbored both adenomas and carcinomas. Frequent loss of heterozygosity was demonstrated on 12q (53.3%), 2p (50.0%), and 18p (50.0%) in adenomas and on 8q (80.0%), 2p (70.0%), 18p (66.7%), and 17p (61.9%) in carcinomas. Significant difference in the loss of heterozygosity rate between the adenoma and the carcinoma was noted on the 17p. Our results suggested that the critical target of loss of heterozygosity in gastric adenoma-carcinoma sequences may be the p53 gene on 17p.

KEY WORDS: Allelotype, loss of heterozygosity, gastric adenoma, carcinoma.