Published in Cancer Detection and Prevention 2001; 25(3):241-246.

Differential Expression of Metallopanstimulin/ S27 Ribosomal Protein in Hepatic Regeneration and Neoplasia

Daniel R. Ganger, MD,a Paul D. Hamllton,b Dennis J. Klos,b Shriram Jakate, MD,c Lawrence McChesney, MD,d and J. Alberto Fernandez Pol, MDb

aSection of Hepatology, Rush Presbyterian St. Luke's Medical Center, Chicago, Illinois, the bLaboratory of Molecular Oncology, DVA Medical Center, SI Louis, Missouri, cDepartment of Pathology, Rush Presbyterian St Luke’s Medical Center, Chicago, Illinois, and dSection of Transplant Surgery, Rash Medical College, Chicago, Illinois.

Address all correspondence and reprint requests to: Address correspondence and reprint requests to: Daniel R. Ganger, MD, University Hepatologists, 1725 W. Harrison #306, Chicago, IL 60612.

ABSTRACT: We have previously shown that human metallopanstimulin-1 (MPS-1) is a ubiquitous 9.4-kd multifunctional ribosomal S27/nuclear "zinc finger" protein that is expressed at high levels in a wide variety of actively proliferating cells and tumor tissues. In this study, we examined the expression of MPS-1 in chronic hepatitus, cirrhosis, and hepatocellular carcinoma. Tissue samples were obtained at the time of tumor resection, needle biopsy, or liver transplantation. MPS-1 was studied by immunohistochemistry by use of specific antibodies to the N-terminus of MPS-1 in a biotin/streptavidin-amplified system. In chronic hepatitus, hepatocytes had very weak MPS-1 immunostaining. In contrast, hepatocytes in regenerating cirrhotic nodules stained strongly for MPS-1. In well-differentiated hepatocellular carcinoma, MPS-1 presence was intense at the periphery of the malignant nodule. In poorly differentiated hepatocellular carcinoma, MPS-1 presence was notably intense in malignant hepatocytes invading the septal tissues, in close contact with neovascular structures These results suggest that MPS-1 may be involved in both progression toward malignancy in regenerating cirrhotic nodules and in subsequent steps of hepatocarcinogenesis.

KEY WORDS: Metallopanstimulin, zinc finger proteins, ribosomal S27 protein, tumor markers, hepatocellular carcinoma.