Published in Cancer Detection and Prevention 2000; 24(6):620-627.

Deletion Mapping on the Short Arm of Chromosome 1 in Merkel Cell Carcinoma

J. Helen Leonard, PhD,a Anthony L. Cook, BSc,a Derek Nancarrow, PhD,b Nicholas Hayward, PhD,c Mireille Van Gele, BSc,d Nadine Van Roy, PhD,d and Frank Speleman, PhDd

aQueensland Radium Institute Laboratory and the cQueensland Cancer Fund Research Unit, Joint Experimental Oncology Program, Queensland Institute of Medical Research, Herston, Queensland, Australia; bQueensland Centre for Schizophrenia Research, Wolston Park Hospital, Brisbane, Queensland, Australia; dCentre for Medical Genetics, University Hospital Ghent, Ghent, Belgium

Address all correspondence to: J.H. Leonard, PhD, QRI Lab, Queensland Institute of Medical Research, Post Office, Royal Brisbane Hospital. Herston Road, Herston, Queensland, Australia 4029.

ABSTRACT: Twenty-two Merkel cell carcinoma (MCC) biopsies and six cell lines from 24 patients were examined for loss of heterozygosity (LOH) at 11 loci on 1p and one on 1q, to determine LOH regions on chromosome 1p. Sixteen (73%) tumors had LOH for at least one locus; 14 demonstrated LOH at more than one locus, and 7 (29%) samples had more than one region of loss, with 4 of these having loss at all informative loci on 1p. Three common regions of loss (SRO) were defined by LOH in multiple tumors. Eight samples demonstrated LOH between D1S214 and DlS16O (1p36), seven between D1S234 and D1S186 (1p35), and 11 for the region centromeric of D1S211 and D1S220 (1p32-1p33). Seven samples (29%) demonstrated more than one region of loss. LOH on 1p occurs frequently in MCC and more than one tumor suppressor gene on 1p is likely to play a role in the development of this tumor type.

KEY WORDS: Merkel cell carcinoma, neuroendocrine, chromosome 1, allelic imbalance.