Published in Cancer Detection and Prevention 2000; 24(6):531-535.

Quantitative Detection of Tumor M2-Pyruvate Kinase in Plasma of Patients with Lung Cancer in Comparison to Other Lung Diseases

Joachim Schneider, MD,a Harald Morr PhD,b Hans-Georg Velcovsky, PhD,c Günter Weisse, PhD,d and Erich Eigenbrodt, PhDe

aInstitut und Poliklinik für Arbeits und Sozialmedizin, Giessen; bPneumologische Klinik Waldhof-Elgershausen, Greifenstein; cMedjzinische Klinik il des Klinikums, Giessen; dInstitut für Toxikologie, Merck KGaA, Darmstadt; and eInstitut fur Biochemie und Endokrinologie, Justus-Liebig Universität, Giessen, Germany

Address all correspondence and reprint requests to: Joachim Schneider, MD, Institut und Poliklinik für Arbeits-und Sozialmedizin, JustusLiebig Universität Giessen, Aulweg 129/III, 0- 35385, Giessen, Germany.

ABSTRACT: Lung cancer is one of the predominant causes of cancer death. The aim of this project is the development of a screening method in persons with high risk for developing lung cancer, based on the measurement of Tumor M2-pyruvate kinase (Tumor M2-PK). Tumor M2-PK is quantitatively detectable in ethylenediaminetetraacetic acid—plasma with a sensitive enzyme-linked immunosorbent assay. So far, 60 patients with newly diagnosed lung cancer were included. These were compared to 24 patients with acute inflammatory lung diseases, 56 patients with pneumoconiosis, 22 patients with obstructive airway diseases, and 28 healthy persons. Tumor patients and some individuals suffering from severe inflammatory lung diseases had significantly higher Tumor M2-PK concentrations in ethylenediaminetetraacetic acid—plasma than all the other groups. The histologic tumor type had no influence on the plasma levels of Tumor M2-PK. Tumor M2-PK concentrations correlate strongly with the tumor stage, showing significantly increasing concentrations with progressive tumor stages. The present data indicate that Tumor M2-PK could be a valuable tumor marker for the detection of lung cancer.

KEY WORDS: ethylenediaminetetraacetic acid-plasma, lung cancer tumor Tumor M2-pyruvate kinase.