Cancer Detection and Prevention Volume 24 / Issue 5 (Sep-Oct 2000)

Table of Contents and Editor's Notes

The peer review process occasionally results in approval of controversial publications that do not necessarily reflect the viewpoint of the editors. Readers of the journal are encouraged to critically review and comment on presented data by submitting a "Letter to the Editor" that may be reprinted in a subsequent issue.

Antioxidant Compounds Interfere with the 3-[4,5-Dimethylthiazol-2-yI]-2, 5-Diphenyltetrazolium Bromide Cytotoxicity Assay

Mohan Natarajan, PhD, Sumathy Mohan, PhD, Bridgetta R. Martinez, BS, Martin L. Meltz, PhD, and Terence S. Herman, MIY

Interpretation of the MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide) assay for cell viability was affected by reduced blue formazan production, resulting from the addition of antioxidants and chemotherapeutic compounds that contain free thiol groups. >>>

Conformational Heterogeneity of P-Glycoprotein

Katalin Goda, MSc, Henrietta Nagy, MSC, László Bene, PhD, Margit Balázs, PhD, Robert Arced, MD, PhD, Eugene Mechetner, PhD, and Gábor Szabó, MD, PhD

The percentage of P-glycoprotein epitiopes available for labeling with UIC2 monoclonal antibody increased significantly (150%-200%) after methanol permeability-fixation of cells. >>>

Interleukin-12-Related Cytokine Gene Expression in Carcinomas of the Breast, Lung, and Larynx: A Study at Tissue Level

Domenico Vitolo, MD, Luciano Ciocci MD, Paola Ferrauti, Francesca Tiboni, Elena Cicerone, MD, Andrea Gab, MD, Marco De Vincentlis, MD, and Carlo D. Baroni, MD

In ductal breast carcinomas, IL-12 and IFN-γ was demonstrated in all cases, and IL-4 in three cases. In non-small cell carcinoma of the lung IL-12 gene expression was observed in 15, IFN-γ in 13 and IL-4 in nine cases. In carcinoma of the larynx, IL-12 was expressed in five, IFN-γ in 9, and IL-4 in five cases. >>>

17 Alpha Androstenediol Inhibition of Breast Tumor Cell Proliferation in Estrogen Receptor-Positive and -Negative Cell Lines

Phuong N. Huynh, PhD, Walter H. Carter, Jr., PhD, and Roger M. Loria, PhD

The antiproliferative effect of 17α-AED (androstene-3β, 17α-diol) measured by tritiated thymidine incorporation was statistically significant and independent of either estrogen or androgen receptors. The 17α-AED treatment of ZR 75-1 (ER+) cells and of the MDA-MB 231 (ER-) cell line both led to reduced cell proliferation. >>>

Neoadjuvant Tamoxifen for Operable Breast Cancer: A Need for Phase III Studies?

Pascal Pujol, MD, PhD, Jean Pierre Daures, MD, PhD, Philippe Rouanet, MD, PhD, Jean Hermand, MD, Jacques Domergue, MD, Jean Grenier, PhD, and Thierry Maudelonde, MD, PhD

The percentage of estrogen receptor positive (ER+) tumors and the mean and median ER levels were lower in the neoadjuvant tamoxifen treated group than in the control groups. There was no statistically significant difference in relapse-free survival between the tamoxifen and control groups. >>>

Implications for Improved High-Dose Methotrexate Therapeutic Effects in Cultured Human Breast Cancer and Bone Marrow Cells

Donnell Bowen, PhD, William M. Southerland, PhD, Donna H. Johnson, MS, Morris Hawkins, Jr., PhD, and Doris E. Huqhes, DVM

Methotrexate (MTX), or the combination of MTX and 5-FU had a greater inhibitory effect on breast cancer cells than trimetrexate (TMQ) or TMQ and 5-FU. The combination of 5-FU and MTX was sequence dependent in bone marrow. A non-toxic does of 5-FU before a high dose of MTX appeared to sustain MTX cytotoxicity in breast cancer and protected against MTX toxicity to bone marrow progenitor cells >>>

Total Sialic Acid Content in Endometrial Cancer Tissue in Relation to Normal and Hyperplastic Human Endometrium

AIicja Paszkowska, PhD, Marek Cybuiski, MD, Andrzej Semczuk, MD, PhD, Krzysztof Postawski MD, PhD, and Henryk Berbec, MD, PhD

The mean tissue total sialic acid (TSA) content in endometrial cancer patients was significantly higher than in the control groups in both the proliferative and secratory phase. In healthy women the tissue TSA content in the secretory phase was higher than in the proliferative phase of the menstrual cycle. >>>

Abnormal G-Actin Content in Single Prostate Cells as a Biomarker of Prostate Cancer

George P Hemstreet, III, MD, PhD, Rebecca B. Bonner, MS, CT Robert E. Hurst, PhD Donna Bell, BS and Barbara L. Bane, MD

In cells collected by fine needle aspiration (FNA) from dominant suspicious nodules of prostatectomy specimens an abnormal G-Actin content was found in 22 of 27 specimens. The abnormal G-Actin content was also identified as a malignancy associated change in cells from adjacent grossly normal areas in five of 22 specimens. >>>

Longitudinal Gompertzian Analysis of Mortality from Prostate Cancer in Japan, 1955-1996

Yoko Imaizumi DSc

In Japan, the recent rising mortality from prostate cancer appeared to be related to rapidly changing lifestyles. The intake of animal proteins correlated most strongly with the rising mortality rates. >>>

Synergistic Potentiating Effect of D(+)- Mannose, Orotic, and Hippuric Acid Sodium Salt on Selective Toxicity of a Mixture of 13 Substances of the Circulatory System in Culture for Various Tumor Cell Lines

Gyula Kulcsár, PhD

Based upon the absence of tumor development in most individuals during their lifetimes, different substances in the circulatory system were investigated and designated as PADS (Passive Antitumor Defense System). Previously, 13 substances were identified to have significant toxic effects on various tumor cell lines in vitro and in vivo when acting synergistically without affecting normal cells. Three of 17 additional substances in the circulatory system were found to be also part of the defense system. >>>

Association between Apolipoprotein E Genotypes and Cancer Risk in Patients with Acquired Immunodeficiency Syndrome

Knut Liestøl, PhD, Eli-Anne Kvittingen, PhD, Helge Rootwelt, MD, Oona Dunlop, MD, Anne K. Goplen, MD, Jan C. Pedersen, MD, Sverre H. Brorson, PhD, Anne-Lise Børresen-Dale, PhD, Bjørn Myrvang, MD, and Jan Mæhlen, MD

In 197 patients with acquired immunodeficiency syndrome (AIDS), 71 with lymphoma or Kaposi sarcoma had a significantly higher frequency of apo-E4 than apo E2 alleles. The authors suggest that the apoE genotype has a marked effect on cancer development in AIDS patients >>>


Herbert E. Nieburgs, MD
Worcester, MA