Published in Cancer Detection and Prevention 2000; 24(Supplement 1).

Frequent allelic loss on chromosome 16p133 in human anaplastic thyroid carcinoma

Y Tamaki MD, M Kadota MD, Y Komoike MD, M Monden MD

Dept Surgery and Clinical Oncology, Osaka University Graduate School of Medicine, Osaka, Japan,

AIM: To investigate the genomic alteration that may responsible for anaplastic transformation of thyroid cancer, loss of heterozygosity (LOH) in tissue samples and cell line samples of thyroid cancers were examined. METHODS: Eight anaplastic and three well-differentiated thyroid cancer cell line samples were screened for gain and loss of DNA copy numbers using comparative genomic hybridization (CGH) method. Then, seven anaplasitc and eight well-differentiated thyroid cancer tissue samples were examined for LOH using several microsatellite markers on 16p and 18q. RESULTS: CGH showed frequent loss of 16p, 18q and gain of 20q in cell lines. Especially, loss of 16p was specifically found in anaplastic cancer cell lines. LOH was found on 16p in all anaplastic thyroid cancer specimens, and the common allelic loss was determined on 16p13.3. No well-differentiated thyroid cancer specimen showed any LOH of the microsatellite markers examined. CONCLUSIONS: We suspect that chromosome 16p13.3 may harbor some tumor suppressor gene responsible for anaplastic transformation, and continue further analysis both of chromosome 16p and 18q.

KEY WORDS: undifferentiated thyroid carcinoma, genetic alteration.

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Paper presented at the International Symposium on Impact of Biotechnology on Cancer Diagnostic & Prognostic Indicators; Geneva, Switzerland; October 28 - 31, 2000; in the section on genetic instability.