Published in Cancer Detection and Prevention 2000; 24(Supplement 1).

Onco and suppressor gene expression is a useful markers of chemical carcinogenesis

I Ember PhD, I Kiss MD

Dept Preventive Medicine, Medical University School, Pécs, Hungary,

AIMS: We have investigated different type of chemical carcinogenes "in vivo" experiments, concerning with onco-supressor gene expression. METHODS: CBA/CA inbred mice were treated intraperitoneally by Cyclophosphamide, 7,12-dimethylbenzanthracene and l-nitropyrene as well. After 24-48 hrs later mice were autopsied and total RNA of thymus, spleen and liver was isolated. RESULTS and CONCLUSIONS: RNA slot blotted onto Hybond N + filters and hybridized with chemiluminescently labeled c-Ha-ras, p53, and c-myc onco/suppressor genes. It has been carried out Ha-ras overexpression at this early period, p53 expression was changed too, but c-myc increasing was not significant. It seems to be in this useful, and new "in vivo" model could be detectable significant overexpression of onco/suppressor genes, showing of the initiation of chemical carcinogenesis action on RNA level. Looks like the onco/suppressor gene overexpression could be used as new biomarker of the initiation of chemical carcinogenesis. In further studies will clear the roll of point mutation of above mentioned genes, as the background of overexpression of genes.

KEY WORDS: overexpression, chemical carcinogenesis.

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Paper presented at the International Symposium on Impact of Biotechnology on Cancer Diagnostic & Prognostic Indicators; Geneva, Switzerland; October 28 - 31, 2000; in the section on genetic risk assessment.