ISPO

Published in Cancer Detection and Prevention 2000; 24(1):80-85.

Significance of Serum Type IV Collagenolytic Activities and Gelatinase Levels for Detectionof Metastasis in Murine RCT Sarcoma

Kazuo Ohmori, MD, PhD, Masahiko kanamori, MD, PhD, Kazuo Yudoh, MD, PhD Thketoshi Yasuda, MD, PhD

Department of Orthopaedic Surgery Toyama Medical and PharmaceuticalUniversity, Toyama, Japan.

Address all correspondence to K Ohmori M D Ph D , Department of Orthopaedic Surgery Toyama Medical and PharmaceuticalUniversity, 2630 Sugitani, Toyama 930-0194, Japan. Tel: +81-76-434-2281, ext 3036. Fax: +8l-76-434-5035

ABSTRACT We investigated the usefulness of serum type IV collagenolytic activities and gelatinase levels asdiagnostic markers of metastasis in the animal model of spontaneous lung metastasis by FITC-labeled type IV col-lagen degradation assay and zymographic analyses. High-metastatic RCT(+) and low-metastatic RCT(—) cloneswere used in the present study. The mean serum type IV collagenolytic activity in the RCT(+) group started toincrease from two weeks after hind limb amputation, and was 0.45 and 1.29 unitlml at three and four weeks. Thesevalues were significantly higher than those in the control group (p <0.01 at three weeks; p < 0.001 at four weeks).A correlation between the number of lung nodules and serum type IV collagenolytic activities in the RCT(+) groupwas found (r 0.89, p <0.001). Zymographic analyses indicated that 105-k.D gelatinolytic activities of the RCT(+)group were higher than those of the RCT(—) group at three and four weeks. Thus, type IV collagenolytic activitiesand serum gelatinase levels might be valuable tnarkers for the detection of metastasis.

KEY WORDS: murine sarcoma, serum type IV collagenolytic activity, serum gelatinase level, lung.

For more information, contact ohmori@ms.toyama-mpu.ac.jp

http://www.cancerprev.org/Journal/Issues/24/1/364