Published in Cancer Detection and Prevention 1999; 23(5):401-407.

Image Cytometric DNA Analysis and PCNA Expression in Transitional Cell Carcinoma of the Bladder

Anna G. Ioakim-Liossi, M.D.,a Peter J. Karakitsos, M.D.,b Dimitrios Pantazopoulos, M.D.,c Kiriaki G. Aroni, M.D.,a and Kalliopi A. Kyrkou, M.D.b

Departments of a Pathology and c Urology, Medical School University of Athens, Athens, Greece, and b Department of Clinical Cytology and Cytogenetics, Laiko Hospital, Athens, Greece

Address all correspondence and reprint requests to: Anna G. Ioakim-Liossi MD, Filadelfeos 32, 145 52 Kefalari Athens Greece.

ABSTRACT: The proliferation rate as determined by PCNA immunostaining and the DNA ploidy status as measured by static cytometry were studied in 70 transitional cell carcinomas or the bladder (TCCB) in relation to grade, stage, atid recurrence. The follow-up period was 2 years. A significant difference was observed in PCNA expression among grades I, II, and III (p <0.02), between superficial (pTa-pT1) and invasive (pT2-pT4) tumors (p < 0.04), between recurring and non-recurring tumors (p < 0.001), and between tumors of the same grade with and without recurrence (p < 0.05). A significant difference was also found in the ploidy pattern among grades I, II, and III (p = 0.002), and between superficial and invasive (p = 0.02) and recurring and non-recurring tumors (p < 0.01). Finally, the recurrence status seems to be strongly influenced by the proliferation rate and ploidy of TCCB (p < 0.001). Our results suggest that the above-studied parameters may offer useful information on the biological behavior of TCCB.

KEY WORDS: transitional cell bladder cancer, DNA ploidy, proliferating cell nuclear antigen, cyclin, WHO grade, stage.