Published in Cancer Detection and Prevention 1998; 22(6):516-525.

Screening for In Vitro Anti-Tumor-Promoting Activities of Edible Plants From Indonesia

Akira Murakami, Ph.D.,a Hideaki Morita, B.S.,a Ratu Safitri, M.S.,b Aseng Ramlan, Ph.D.,b Koichi Koshimizu, Ph.D.,a and Hajime Ohigashi, Ph.D.c

a Department of Biotechnological Science, Faculty of Biology-Oriented Science and Technology, Kinki University, Iwade-Uchita, Wakayama, Japan; b Department of Biology, Faculty of Mathematics and Natural Sciences, Padjadjaran University, Singaperbangsa, Bandung, Indonesia; and c Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan

Address all correspondence and reprint requests to: Hajime Ohigashi, Ph.D., Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto 606-01, Japan.

ABSTRACT: A total of 135 methanol extracts from 48 plant families (107 species) of edible Indonesian plants were screened for their in vitro anti-tumor-promoting activities using the tumor promoter 12-O-hexadecanoylphorbol-13-acetate (HPA)-induced Epstein-Barr virus (EBV) activation test in Raji cells. Examined at a concentration of 200 mg/ml, 71% of the extracts inhibited EBV activation by 30% or more. The rate is comparable to the rate observed in our previous tests of edible Thai (60%) plants and much higher than the rate (26%) observed with Japanese plants. In particular, the plant families Zingiberaceae (13/13 active) and Umbelliferae (7/8 active) are suggested to be desirable sources of effective cancer-preventive agents because of the strikingly high frequency of inhibitory activity. Thus, a high potential of edible Southeast Asian plants for cancer chemoprevention is indicated.

KEY WORDS: chemoprevention, Epstein-Ban virus, Southeast Asian vegetables and fruits, Umbelliferae, Zingiberaceae.

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