Published in Cancer Detection and Prevention 1997; 21(6):553-562.

Effects of Catechols on Free Radical Formation by Chemotherapeutic Agents (Adriamycin, Farmorubicin, and Mitomycin)

Michel Bounias DSca,b, Jozef Kladny PhDc, Irena Kruk PhDd, Teresa Michalska PhDd, Krzysztof Lichszteld PhDd

aDepartment of Phytopharmacy and Ecotoxicology, University of Avignon and INRA, Versailles, France; bResearch Department, Alexandria Institute of Medicine, Hastings-on-Hudson, NY; cClinic of General and Vascular Surgery, Pomeranian Medical Academy, Szczecin, Poland; dInstitute of Physics, Technical University of Szczecin, Szczecin, Poland

Address all correspondence and reprint requests to: Michel Bounias, D.Sc., Unite de Biomathematique et Toxicologie, Universite d'Avignon, Faculte des Sciences, 33 rue Pasteur F 84000 Avignon, France.

ABSTRACT: The aim of the present study was to investigate the effects of biologically important catechols on the cytotoxicity of adriamycin, farmorubicin, and mitomycin C with respect to hydroxyl radical production. Catecholamines (adrenaline, noradrenaline, dopamine) and DOPA enhance the generation of hydroxyl radicals by chemotherapeutic antibiotics. Measures were done using a deoxyribose assay, in presence of the Co(II) + H2O2 system. Catalase and hydroxyl radical scavengers (mannitol, thiourea, cysteine, glutathione, L-lactic dyhydrogenase) inhibited the deoxyribose damage caused by the drugs.

KEY WORDS: adriamycin, catecholamines, farmorubicin, hydroxyl radical, mitomycin C.

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