ISPO

Published in Cancer Detection and Prevention 1997; 21(2):196-200.

Synaptonemal Complex Damage in Mouse Spermatocytes Exposed to Tequila and Brandy

A. Pina-Calva, MS, R. Quezada-Medina, Biol., and E. Madrigal-Bujaidar, DS

Genetics Laboratory, National School of Biological Sciences, I.P.N. Carpio y Plan de Ayala, Mexico City, Mexico

Address all correspondence and reprint requests to: Eduardo Madrigal-Bujaidar, DS., Laboratorio de Genetica, Escuela Nacional de Ciencias Biologicas, I.P.N. Carpjo y Plan de Ayala, Sto. Tomas Mexico, D.F. C.P. 11340, Mexico.

ABSTRACT: We evaluated the capacity of tequila and brandy to induce synaptonemal complex (SC) breaks in mouse spermatocytes. The alcoholic beverages were 20% diluted in distilled water and administered daily by oral intubation for 21 days (1, 2, and 3 g/kg, six animals per group). A positive control group was administered cyclophosphamide (CP) (20 mg/kg), and another group of mice was treated with distilled water. The results indicated the following: (i) tequila induced a statistically significant increase in SC damage with 2 g/kg and 3 g/kg, and brandy was genotoxic only with 3 g/kg (ii) CP induced almost a duplication of the total number of breaks produced by tequila or brandy; (iii) the average weight increase in the animals was 3.3 g, but the group treated with the highest dose of tequila showed a slight weight decrease; (iv) the weight of the testes did not show any significant difference among groups of animals; and (v) there were no significant differences between groups with respect to the frequency of pachytenes (mean number: 195 in 1000 cells).

KEY WORDS: synaptonemal complex, mouse spermatocytes, tequila, brandy.

http://www.cancerprev.org/Journal/Issues/21/2/188