Published in Cancer Detection and Prevention 1997; 21(2):167-177.

Detection of Melanoma by Monitoring the Intracellular Fluorescein Fluorescence Polarization Changes in Lymphocytes

Merimsky, MD, M.Oncol.a,. B. Kaplan, MDb, M. Deutsch, PhDc, R. Tirosh, Ph Dc, A. Weinreb, PhDc, and S. Chaitchik, MDa

aDepartment of Oncology, Tel Aviv Sourasky Medical Center and Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel; bDepartment of Dermatology, Sheba Medical Center, Tel Hashomer, and Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel; and cDepartment of Physics, Jerome Schottenstein Cellscan Center for Early Detection of Cancer, Bar-Ilan University, Ramat-Gan, Israel

Address all correspondence and reprint requests to: Ofer Merimsky, M.D Department of Oncology, Tel Aviv Sourasky Medical Center, 6 Weizman St., Tel Aviv 64239, Israel.

ABSTRACT: The effectiveness of detecting melanoma by measuring the intracellular fluorescein fluorescent polarization (IFFP) of patients' SCM (structuredness of the cytoplasmic matrix)-responding lymphocytes was examined. SCM-responding lymphocytes from 46 melanoma patients and 32 healthy volunteers were labeled with fluorescein diacetate and challenged with different stimuli, and the resulting polarization was determined. The polarizations (P) obtained upon stimulation with nothing (P-O), encephalitogenic factor (P-EF), phytohaemagglutinin (P-PHA), or melanoma antigen (P-MEL), and the ratios RR(ef) (P-EF/P-PHA) and RR(mel) (P-MELIP-PRA) were lower for SCM-responding lymphocytes from the patients as a group than for those of the controls. The specificity and sensitivity of the IFFP tests (using cutoff values) to detect melanoma were 90.6 and 73.9%, respectively. The IFFP tests may facilitate the discrimination between melanoma patients and healthy subjects, and may be used in follow-up of patients with melanoma.

KEY WORDS: intracellular fluorescein fluorescence polarization, diagnosis, follow-up, melanoma.