ISPO

Published in Cancer Detection and Prevention 1997; 21(1):51-54.

Immunoreactive Adrenomedullin in Human Adrenal Glands and Adrenal Tumors

Fumitoshi Satoh1, Kazuhiro Takahashi2, Osamu Murakami1, Fumitoshi Totsune1, Masahiko Sone1, Makoto Ohneda1, Keishi Abe1, Yukio Miura3, Toraichi Mouri4

1Second Department of Internal Medicine; 2Department of Applied Physiology and Molecular Biology; 3Health Center; (4(Second Department of Pathology, Tohoku University School of Medicine, Sendai, Japan

Address all correspondence and reprint requests to: Kazuhiro Takahashi, Department of Applied Physiology and Molecular Biology, Tohoku University School of Medicine, 2-1 Seiryo-cho, Aoba-ku, Sendai, Miyagi 980-77, Japan.

ABSTRACT: Adrenomedullin is a potent vasodilator peptide that was isolated from human pheochromocytoma. We developed a sensitive and specific radioimmunoassay for adrenomedullin and studied the presence of adrenomedullin in human adrenal glands and adrenal tumors, including pheochromocytoma. High concentrations of immunoreactive adrenomedullin were found in normal parts of adrenal glands (cortex and medulla) (12.6 +/- 1.0 pmol/g wet wt, N = 7 mean +/- SEM). High concentrations of immunoreactive adrenomedullin were also present in the tumor tissues of pheochromocytoma (4.5 +/- 1.5 pmollg wet wt, N = 11). Immunoreactive adrenomedullin was detected in some adrenocortical tumors, hut these concentrations were much lower than those in the normal adrenal glands and pheochromocytomas. Reverse phase high- performance liquid chromatography of the normal adrenal gland and pheochromocytoma showed a peak eluting in the position of synthetic adrenomedullin 1-52. The present study has shown the presence of high concentrations of immunoreactive adrenomedullin in the normal adrenal glands and pheochromocytomas.

KEY WORDS: adrenomedullin, adrenal, pheochromocytoma, radioimmunoassay, chromatography, neuropeptide.

http://www.cancerprev.org/Journal/Issues/21/1/169