Published in Cancer Detection and Prevention 1996; 20(1):1-10.

NotI Jumping and Linking Clones as a Tool for Genome Mapping and Analysis of Chromosome Rearrangements in Different Tumors

Eugene R. Zabarovsky, Ph.D.a,b, Vladimir L Kashuba, Ph.D. a,c, Rinat Z. Gizatullin, Ph.D.a, Gosta Winberg, Ph.D.a, Veronika L Zabarovska, M.S.a, Rikard Erlandsson, M.S.a, Dmitry A.Domninsky,Ph.D.b, Vladimir M. Bannikov, Ph.D.b, Ekaterina Pokrovskaya, M.S.a Irina Kholodnyuk, Ph.D. a, Nikolai Petrov, Ph.D.d, Vladimir M. Zakharyev, Ph.D.b, Lev L. Kisselev, Ph.D.a, and George Klein, M.D.a

a Microbiology and Tumor Biology Center, Karolinska Institute, Stockholm, Sweden; b Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia; c Institute of Molecular Biology and Genetics, Ukranian Academy of Science, Kiev, Ukrainia; and d Vector Incorporated, Koltsovo, Novosibirsk, Russia

Address all correspondence and reprint requests to: Eugene R. Zabarovsky, Ph.D., Microbiology and Tumor Biology Center, Karolinska Institute, Box 280, S-171 77 Stockholm, Sweden.

ABSTRACT: Long-range restriction site maps are of central importance for mapping the human genome. The use of clones from linking and jumping libraries for genome mapping offers a promising alternative to the laborious procedures used up until now. In the present review, this research field is analyzed with particular emphasis on the implementation of a shot-gun sequencing strategy for genome mapping and the use of Notl linking clones for analysis of rearrangements in tumors and tumor cell lines.

KEY WORDS: recombinant DNA, shot-gun sequencing strategy, HUGO project, new technologies, sequence tagged sites, human chromosome 3.