Published in Cancer Detection and Prevention 1996; 20(1):1-10.
NotI Jumping and Linking Clones as a Tool for Genome Mapping and Analysis of Chromosome Rearrangements in Different Tumorsa Microbiology and Tumor Biology Center, Karolinska Institute, Stockholm, Sweden; b Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia; c Institute of Molecular Biology and Genetics, Ukranian Academy of Science, Kiev, Ukrainia; and d Vector Incorporated, Koltsovo, Novosibirsk, Russia
Address all correspondence and reprint requests to: Eugene R. Zabarovsky, Ph.D., Microbiology and Tumor Biology Center, Karolinska Institute, Box 280, S-171 77 Stockholm, Sweden.
ABSTRACT: Long-range restriction site maps are of central importance for mapping the human genome. The use of clones from linking and jumping libraries for genome mapping offers a promising alternative to the laborious procedures used up until now. In the present review, this research field is analyzed with particular emphasis on the implementation of a shot-gun sequencing strategy for genome mapping and the use of Notl linking clones for analysis of rearrangements in tumors and tumor cell lines.