Cancer Detection and Prevention Volume 20 / Issue 1 (Jan-Feb 1996)

Table of Contents and Editor's Notes

The peer review process occasionally results in approval of controversial publications that do not necessarily reflect the viewpoint of the editors. Readers of the journal are encouraged to critically review and comment on presented data by submitting a "Letter to the Editor" that may be reprinted in a subsequent issue.

NotI Jumping and Linking Clones as a Tool for Genome Mapping and Analysis of Chromosome Rearrangements in Different Tumors

Eugene R. Zabarovsky, Ph.D., Vladimir L Kashuba, Ph.D. , Rinat Z. Gizatullin, Ph.D., Gosta Winberg, Ph.D., Veronika L Zabarovska, M.S., Rikard Erlandsson, M.S., Dmitry A.Domninsky,Ph.D., Vladimir M. Bannikov, Ph.D., Ekaterina Pokrovskaya, M.S. Irina Kholodnyuk, Ph.D. , Nikolai Petrov, Ph.D., Vladimir M. Zakharyev, Ph.D., Lev L. Kisselev, Ph.D., and George Klein, M.D.

Human genome mapping by use of clones from linking and jumping libraries is extensively reviewed and chromosome rearrangements in various tumors are described. The authors analyze much of their own experience on approaches and strategies for human genome mapping. >>>

Value of Autologous Bone Marrow Transplantation (ABMT) in 42 Patients with Follicular Lymphomas Responsive to Conventional Chemotherapy: A "France Autogreffe" Study

Claude Linassier, M.D., Loic Fouillard, M.D., Noe1 Milpied, M.D., Herve Tilly, M.D., Jose Pico, M.D. Jean Francois Abgrall, M.D.,Bertrand Coiffier, M.D., Raoul Herbrecht, M.D., Pierre Biron, M.D., and Philippe Colombat, M.D.

In a retrospective study about 50% of selected patients with nontransformed follicular lymphoma were grafted with marrow-hematopoietic stem cells in first partial remission or in chemosensitive relapse. After autologous bone marrow transplantation and a median follow-up of 43 months, 60% had a relapse-free survival, 58% had an event-free survival and 83% had overall survival inspite of positive bcl-2 in the peripheral blood. >>>

A Possible Role for Human Papillomaviruses and c-myc, c-Ha-ras, And p53 Gene Alterations in Malignant Cutaneous Lesions From Renal Transplant Recipients

Isabelle Pelisson, Ph.D., Chantal Soler, Ph.D., Yvette Chardonnet, Ph.D., Sylvie Euvrard, M.D., and Daniel Schmitt, Ph.D.

In cutaneous biopsies of sun-exposed areas from 10 renal transplant recipients several years after grafting, HPV DNA was detected in 23 of 34 squamous cell carcinomas (SCC) and potentially oncogenic HPV types 16/18 were found in 16 of 22 cases. The proportion of positive biopsies for HPV using PCR (14/21) was similar to in situ hybridization (16/22). In situ hybridization revealed c-myc gene in 4 of 13 biopsies. While c-Ha-ras gene mutations were rare, p53 protein accumulation was noted in all SCC. >>>

Bone Marrow Stromal Cell Defects and 1-α, 25-dihydroxyvitamin D3 Deficiency Underlying Human Myeloid Leukemias

Istvan Blazsek, D.S., Christian Farabos, M.D., Philippe Quittet, M.D., Marie-Louise Labat, D.S., Annie France Bringuier, Boffa Kim Triana, M.D., David Machover, M.D. Michel Reynes, M.D., and Jean-Louis Misset, M.D.

The hematon multicellular complex of fibroblasts, adipocytes, endothelial cells, macrophages, hematopoietic cobblestone area-forming cells, high potential colony-forming unit, granulocyte-macrophage colony-forming unit, burst-forming unit erythroid and the terminally differentiated cells were present in most bone marrow aspirates from healthy donors. They were absent in patients with primary myelodysplasia and acute myelogenous leukemia in the first perceptible phase and in patients with chronic myelogenous leukemia throughout the disease. >>>

Establishment and Characterization of Two Cell Lines Derived From a Single Hepatocellular Carcinoma Containing Multiploid DNA Distribution

Jen-Hwey Chiu, M.D., Ph.D., Hwey-May Chang, B.S., Hwa-Li Kao, B.S., Li-Hwa Wu, M.S., and Wing-Yiu Lui, M.D.

Two human hepatocellular (HCC) cell lines, HAGS 2.1 and HAGS 2.2 from a single HCC tumor nodule containing a polypoid DNA distribution, were established by primary culture and single cloning methods. The HAGS 2.1 cells were spindle-shaped with a DNA ploidy of 4.4N, while HAGS 2.2 cells were polygonal with 5.1 DNA ploidy. The HAGS 2.1 were less differentiated and grew faster than the relatively better-differentiated HAGS 2.2 cells with doubling times of 38 and 42 h respectively. >>>

Cytokines (IL-6, TNF-α, IL-1α) and Soluble Interleukin-2 Receptor as Serum Tumor Markers in Multiple Myeloma

Xavier Filella, M.D., Joan Blade, M.D., Armando Lopez Guillermo, M.D., Rafael Molina, M.D., Ciril Rozman, M.D., and Antonio M. Ballesta, M.D.

Serum levels of interleukin-1-alpha (IL-1-alpha), tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) and soluble interleukin 2 receptor (sIL-2r) were evaluated as tumor markers in patients with monoclonal gammopathy of undetermined significance (MGUS) and with multiple myeloma (MM). Patients with MGUS and MM had higher serum levels of IL-6 and TNF-alpha than the control group. Patients with progressive disease had higher levels of sIL-2r than normal controls and patients in a stable phase. The authors suggest that these cytokines may play a sole in the pathogenesis of MM. >>>

Urinary 1-hydroxypyrene, A Pah Biomarker in Foundry Workers

O Omland, M.D., D. Sherson, M.D., A. M. Hansen, Ph.D., T. Sigsgaard, M.D., Ph.D., H. Autrup, Ph.D., and E. Overgaard, M.D.

In 70 male workers of an iron foundry exposed to polycyclic aromatic hyrdrocarbons (PAH) and in a control group of 68 male blue-collar corkers, urine samples were tested for 1-hydroxypyrene (HPU) levels. Smoking and nonsmoking foundry workers had significantly higher median HPU concentrations than their control counterparts. The authors conclude that HPU is a sensitive biomarkers for low-dose PAH exposure and suggest that it may be etiologically related to increased risk of lung cancer in foundry workers >>>

Prognostic Value of p53 Protein Expression in Squamous Cell Carcinoma of the Esophagus

Genevieve M. Monges, M.D., Jean-Francois Seitz, M.D., Marc F. Giovannini, M.D., Joanny M. Gouvernet, M.D., Michelle A. M. Torrente, and Jacques A. Hassoun, M.D.

Endoscopic biopsy specimens form 76 patients with histologically proven squamous cell carcinoma of the esophagus (SCCE) were examined by immunostaining with three p52 monoclonal antibodies following two courses of concomitant chemotherapy and radiation therapy (CCR). In p53-negative cases the median survival time was longer (23 months versus 8) and complete responses were greater than in p53-positive cases (20 of 25 versus 16 of 29). The authors suggest that immunohistochemical detection of p53 protein and the tumor diameter may be used to predict the response to CCR. >>>

Activation of Two Mutant Androgen Receptors From Human Prostatic Carcinoma by Adrenal Androgens and Metabolic Derivatives of Testosterone

Zoran Culig, M.D., Jutta Stober, Andreas Gast, Heike Peterziel, Alfred Hobisch, M.D., Christian Radmayr, M.D., Anton Hittmair, M.D., Georg Bartsch, M.D., Andrew C. B. Cato, Ph.D., and Helmut Klocker, Ph.D.

The functional characterization of two mutant receptors in the presence of adrenal androgens and testosterone metabolites is reported. The central regulatory role of the androgen receptor (AR) and its target of androgen ablation therapy in prostatic carcinoma was studied by cotransfecting androgen-responsive receptor genes and either the wild type or mutant AR expression vectors into receptor-negative DU-145 and CV-1 cells and by in vitro electrophoretic mobility shift assays (EMSA) of receptor hormone complexes interaction with target DNA. The authors conclude that mutation of the AR gene, creating a receptor with a broadened ligand specificity seems to be one of the mechanisms that enable tumor cells to circumvent the effect of androgen withdrawal by reactivation of the androgen signal transduction pathway. >>>

Perceived risk, anxiety, Mammogram uptake, And Breast Self-Examination of Women with a Family History of Breast Cancer: The Role of Knowing to Be At Increased Risk

Constance C. H. Drossaert, M.A., Henk Boer, Ph.D., and Erwin R. Seydel, Ph.D.

In a national population-based Dutch breast cancer screening program, risk perception , breast cancer anxiety and early detection behavior was assessed in 389 women with a family history of breast cancer and in 3295 women without a family history. The group with a positive family history was divided into women with awareness of the risk factor and those without. The cases with a negative family history were placed into categories with either having had an experience of breast cancer in their environment or not. It was found that women with a positive family history and those without similarly underestimated the average women' lifetime breast cancer risk. Risk perception in women with a positive family history was comparable to risk perception in women with a negative family history but who had experienced a breast cancer case in their environment. Although knowledge of belonging to a risk category influenced risk perception it did not have an effect on early detection behavior of women with a positive family history of breast cancer. >>>


Herbert E. Nieburgs, MD
Worcester, MA