Published in Cancer Detection and Prevention 1995; 19(3):292-298.

Human Promyelocytic Leukemia Cells (Hl-60): A New Model to Study the Effects of Chemopreventive Agents on h(2)0(2) Production

Ramesh S Bhimani, Ph.D.^a, Zhaojing Zhong, M.D.^a, Eric Schleifer, B.S.^a, Walter Troll, Ph.D.^a, Krystyna Frenkel, Ph.D.^a,b

Address all correspondence and reprint requests to: Krystyna Frenkel, Ph.D., Dept of Environmental Medicine, New York Univ Medical Center, 550 First Avenue, New York, NY 10016-6451

ABSTRACT: Tumor promoter-stimulated polymorphonuclear leukocytes (PMNs) produce excessive H(2)0(2), which contributes to inflammation and carcinogenesis. A new model to study 12-0- tetradecanoylphorbol-13-acetate (TPA)-mediated H(2)0(2) formation and its suppression by chemopreventive agents was developed using human promyelocytic leukemic HL-60 cells and validated by comparing results with those obtained using human PMNs. Equal H(2)O(2) (20 to 25 nmol/ml) was generated by TPA-activated PMNs (2.5 x 10(5)/ml) and TPA-treated dimethylsulfoxide (DMSO)- differentiated HL-60 cells (5 x 10(5)/ml). The chemopreventive agent-mediated inhibition of TPA-induced H202 formation was also comparable in both cell types. These results suggest that HL-60 cells can become a useful in vitro system to screen rapidly for chemopreventive agents and to study their properties.

KEY WORDS: chemoprevention, differentiation, hydrogen peroxide, neutrophils.

http://www.cancerprev.org/Journal/Issues/19/3/77