Published in Cancer Detection and Prevention 1994; 18(3):231-240.

Expression of Transcripts for csf-1 and for the "Macrophage" and "Epithelial" Isoforms of the csf-1 R Transcripts in Human Ovarian Carcinomas

Thomas Bauknecht, MDa, Marion Kiechle-Schwarz, MDa Andreas du Bois, MDa, Jutta Wolflea, Barry Kacinski, PhDb

aDept of Gynecology and Obstetrics, Albert-Ludwigs Univ, 79106 Freiburg, Hugstetterstr. 55, FRG; bYale Univ, Radiobiology Laboratories, 333 Cedar Street, New Haven, CN

Address all correspondence and reprint requests to: Dr. Thomas Bauknecht, Universitats-Frauenklinik, Hugstetterstr. 55, 79106 Freiburg i.Br., Germany

ABSTRACT: Levels of CSF-1 and CSF-1 receptor (CSF-1 R) (fms) transcripts were quantified by Northern and dot blotting in total RNA extracts from 68 human ovarian carcinomas and positive and negative controls. Overall, very high levels of CSF- 1 and CSF- 1 R transcripts were found in 11 and 21 % of ovarian carcinoma specimens, respectively. When probes specific for CSF- 1 R exon I were employed, which hybridize with the "epithelial" but not with the "hematopoietic" isoforms of the CSF-1R transcripts, the results suggested that both isoforms of the CSF-1R transcript were present in the tumor specimens. When levels of CSF-1 and CSF-1R were compared, a positive correlation was observed. Taken together, these results confirm prior observations of high levels of CSF-1 and CSF-1R in ovarian carcinoma specimens and that the latter include both "epithelial" and "macrophage" CSF-1R transcript isoforms, as might be expected for specimens composed of malignant epithelial cells and stromal macrophages.

KEY WORDS: ovarian cancer, oncogenes, growth factors, CSF-1 receptor, fms, isoforms.